[在感染过程动态变化中对患者粪便中甲型肝炎病毒的测定]

[Determination of hepatitis A virus in the feces of patients in the dynamics of the infectious process].

作者信息

Doroshenko N V, Alekseeva I L, Zairov G K, Shakhgil'dian I V, Konstantinova L A

出版信息

Vopr Virusol. 1982 May-Jun;27(3):308-11.

Abstract

Excretion of hepatitis A virus (HAV) in the time course of the infectious process is described. Among 197 fecal specimens collected from 45 patients HAV was found in 40 (20.5%); in 51% of the patients the virus was isolated in the 1st week of the icteric period, in 25% in the 2nd week and in 4% in the 3rd week. The buoyant density in cesium chloride gradient of virus specimens obtained on the 1st-2nd day of jaundice was represented by one peak of 1.30-1.35 g/cm3, whereas the material collected at late periods of the disease had heterogeneous buoyant density in zones from 1.24 to 1.43 g/cm3. The immune electron microscopic studies showed the fecal specimens collected early in the disease to be richer in virus than those collected at later intervals.

摘要

本文描述了甲型肝炎病毒（HAV）在感染过程中的排泄情况。在从45名患者收集的197份粪便标本中，40份（20.5%）检测到HAV；51%的患者在黄疸期第1周分离出病毒，25%在第2周，4%在第3周。黄疸第1 - 2天获得的病毒标本在氯化铯梯度中的浮力密度表现为一个1.30 - 1.35 g/cm³ 的峰，而疾病后期收集的材料在1.24至1.43 g/cm³ 区域具有异质浮力密度。免疫电子显微镜研究表明，疾病早期收集的粪便标本比后期收集的含有更多病毒。