Doroshenko N V, Shevtsova Z V, Stakhanova V M, Lapin B A, Lomovskaia I B, Zairov G K, Krylova R I, Dzhelieva Z N, Anan'ev V A
Vopr Virusol. 1990 Jul-Aug;35(4):299-303.
Hepatitis A infection characterized by virus excretion in feces, synthesis of specific IgM antibody, increased activity of alanine aminotransferase in the blood serum, and a complex of morphological lesions in the liver typical of acute hepatitis was reproduced in M. fascicularis (M. f.) and Macaca rhesus (M. r.) using 2 strains of hepatitis A virus (HAV) isolated from human patients. The incubation period varying from 9 to 23 (mean 16) days in M. f. and from 12 to 35 (mean 18) days in M. r. in primary infection shortened to 1-12 (mean 10) and 3-6 (mean 5) days in the process of virus passage from monkey to monkey. The disease was observed to run both manifest forms (except jaundice) typical of human HA and an inapparent form in which the level of enzymes remained within normal limits but HAV could be detected in feces, anti-HAV-IgM in the blood serum, and morphologically acute hepatitis in the liver. Immune electron microscopy of both the initial material and in monkey feces at the levels of all three passages revealed complexes consisting of spherical viral particles 27-29 nm in size coated with antibodies. The immune complexes formed upon addition to the fecal extracts under study of IgG isolated both from human convalescent sera and from sera of experimentally infected monkeys collected in the acute stage of the illness.
利用从人类患者分离出的2株甲型肝炎病毒（HAV），在食蟹猴（M. f.）和恒河猴（M. r.）身上再现了甲型肝炎感染，其特征为粪便中排出病毒、合成特异性IgM抗体、血清中丙氨酸转氨酶活性增加以及肝脏出现典型急性肝炎的一系列形态学病变。在初次感染时，食蟹猴的潜伏期为9至23天（平均16天），恒河猴为12至35天（平均18天）；在病毒在猴与猴之间传代过程中，潜伏期缩短至1至12天（平均10天）和3至6天（平均5天）。观察到该疾病既有人类甲型肝炎典型的显性形式（除黄疸外），也有隐性形式，即酶水平保持在正常范围内，但粪便中可检测到HAV，血清中可检测到抗HAV-IgM，肝脏出现形态学上的急性肝炎。对初始材料以及所有三代传代猴粪便进行免疫电子显微镜检查，均发现由大小为27至29纳米的球形病毒颗粒与抗体结合形成的复合物。这些免疫复合物是在将从人类恢复期血清以及患病急性期实验感染猴血清中分离出的IgG添加到所研究的粪便提取物后形成的。
