ftsB基因作为大肠杆菌中nrdB基因一个等位基因的特性分析。
Characterization of the ftsB gene as an allele of the nrdB gene in Escherichia coli.
作者信息
Kren B, Fuchs J A
出版信息
J Bacteriol. 1987 Jan;169(1):14-8. doi: 10.1128/jb.169.1.14-18.1987.
Abstract
A temperature-sensitive, salt-rescuable ftsB cell division mutant, MFT84, was found to be hydroxyurea sensitive on low-salt medium. Complementation studies with plasmids and a marker rescue study with bacteriophage M13 nrd indicated that ftsB is an allele of nrdB and that the mutation occurs in the region corresponding to nucleotides 6729 to 7032 of the nrdB gene. Enzymatic characterization demonstrated that the B2 subunit of ribonucleoside-diphosphate reductase encoded by ftsB was responsible for the decreased activity and the thermolability of the enzyme. The ftsB-encoded B2 subunit was activated by the addition of 0.1 M NaCl to an in vitro assay, corroborating the in vivo temperature-dependent salt requirement was a result of a defective B2 subunit.
摘要
发现一个温度敏感、盐可拯救的ftsB细胞分裂突变体MFT84在低盐培养基上对羟基脲敏感。用质粒进行的互补研究和用噬菌体M13 nrd进行的标记拯救研究表明，ftsB是nrdB的一个等位基因，且该突变发生在nrdB基因对应于核苷酸6729至7032的区域。酶学特性表明，ftsB编码的核糖核苷二磷酸还原酶的B2亚基导致了该酶活性的降低和热不稳定性。在体外试验中，添加0.1M NaCl可激活ftsB编码的B2亚基，这证实了体内温度依赖性盐需求是有缺陷的B2亚基造成的结果。
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