Riley M, O'Reilly C, McConnell D
J Bacteriol. 1984 Feb;157(2):655-7. doi: 10.1128/jb.157.2.655-657.1984.
More than 55 kilobases of chromosomal DNA of Salmonella typhimurium LT2, including the gpt, proA, ataA, and newD genes, were cloned in plasmid vector pULB113. The locations of the genes and selected restriction endonuclease cleavage sites were established, and some of the restriction enzyme fragments were subcloned in plasmid vector pBR322.
鼠伤寒沙门氏菌LT2超过55千碱基对的染色体DNA,包括gpt、proA、ataA和newD基因,被克隆到质粒载体pULB113中。确定了这些基因的位置以及选定的限制性内切酶切割位点,并且一些限制性酶切片段被亚克隆到质粒载体pBR322中。