逆转录病毒DNA的长末端重复序列（LTR）衍生重组：携带从LTR到gag区域倒置的狒狒内源性病毒DNA异常克隆的序列分析。
Long terminal repeat (LTR)-derived recombination of retroviral DNA: sequence analyses of an aberrant clone of baboon endogenous virus DNA which carries an inversion from the LTR to the gag region.
作者信息
Tamura T, Takano T
出版信息
Nucleic Acids Res. 1982 Sep 11;10(17):5333-43. doi: 10.1093/nar/10.17.5333.
Abstract
Nucleotide sequences of a cloned proviral DNA of baboon endogenous virus M7 were analyzed, which carried an internal inversion. The inversion of 2.2 kilobase pairs was occurred between the junction of two tandem LTRs and a site locating in the p30 region of the gag gene. The ATAA sequence was a target for recombination generating the inversion, which was duplicated at both ends of the inverted segment. AAA and CA were lost at the 5'- and 3'-ends of the LTRs by the inversion, respectively. On both sides of the target sequence, long AG-rich stretches were detected, which may specify the site of recombination together with the target sequence. The characteristic base changes in the inversion are concluded to result from an illegitimate recombination associated with LTRs, as well as in case of provirus integration into the host cell DNA. We propose and discuss models to explain the processes of recombination to generate both inversion and integration.
摘要
对狒狒内源性病毒M7的克隆前病毒DNA的核苷酸序列进行了分析，该序列存在一个内部倒位。2.2千碱基对的倒位发生在两个串联LTR的连接处与位于gag基因p30区域的一个位点之间。ATAA序列是产生倒位的重组靶点，该靶点在倒位片段的两端重复出现。LTR的5'端和3'端分别因倒位而丢失了AAA和CA。在靶序列两侧检测到富含AG的长片段，其可能与靶序列一起确定重组位点。倒位中特征性的碱基变化被认为是由与LTR相关的非法重组导致的，就像前病毒整合到宿主细胞DNA的情况一样。我们提出并讨论了解释产生倒位和整合的重组过程的模型。
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