巴氏甲烷八叠球菌中甲醇：2-巯基乙烷磺酸甲基转移酶的激活与失活
Activation and inactivation of methanol: 2-mercaptoethanesulfonic acid methyltransferase from Methanosarcina barkeri.
作者信息
van der Meijden P, Heythuysen H J, Sliepenbeek H T, Houwen F P, van der Drift C, Vogels G D
出版信息
J Bacteriol. 1983 Jan;153(1):6-11. doi: 10.1128/jb.153.1.6-11.1983.
Abstract
Methanol is converted to methane by crude extracts of Methanosarcina barkeri. The first reaction involved in this process, is catalyzed by methanol:2-mercaptoethanesulfonic acid methyltransferase (EC 2.1.1.-). The methyltransferase has an optimum at pH 6.5 and is not inhibited by 2-bromoethanesulfonic acid. Pyridoxal-5'-phosphate acts as an inhibitor (Ki = 0.30 mM). The methyltransferase was tested in the presence of 2-bromoethanesulfonic acid, which inhibits the conversion of 2-(methylthio)ethanesulfonic acid to methane. The reaction is subject to activation and inactivation. Inactivation is brought about by the presence of oxygen, flavin mononucleotide, flavin adenine dinucleotide, and 2-(methylthio)ethanesulfonic acid, the product of the reaction. Activation of the system requires the presence of ATP and Mg2+ and of hydrogen. Hydrogen can be replaced by enzymatic systems, such as pyruvate dehydrogenase, which deliver free hydrogen.
摘要
巴氏甲烷八叠球菌的粗提取物可将甲醇转化为甲烷。该过程涉及的第一个反应由甲醇：2-巯基乙烷磺酸甲基转移酶（EC 2.1.1.-）催化。甲基转移酶在pH 6.5时活性最佳，且不受2-溴乙烷磺酸抑制。磷酸吡哆醛-5'-磷酸作为抑制剂（Ki = 0.30 mM）。在2-溴乙烷磺酸存在的情况下对甲基转移酶进行了测试，2-溴乙烷磺酸会抑制2-（甲硫基）乙烷磺酸向甲烷的转化。该反应会发生激活和失活。失活是由氧气、黄素单核苷酸、黄素腺嘌呤二核苷酸以及反应产物2-（甲硫基）乙烷磺酸的存在导致的。该系统的激活需要ATP、Mg2+和氢气的存在。氢气可用诸如丙酮酸脱氢酶等能提供游离氢的酶系统替代。
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