Ellefson W L, Wolfe R S
J Biol Chem. 1980 Sep 25;255(18):8388-9.
A major simplification of the methyl coenzyme M methylreductase system of Methanobacterium has been effected. The 500,000-dalton hydrogenase complex has been replaced by an NADPH-coenzyme F420 oxidoreductase. By use of this electron-generating reaction, the methylreductase was found to be localized in component C, an acidic protein fraction. In the presence of the oxidoreductase and the methylreductase, formation of methane under a nitrogen atmosphere was dependent upon the addition of NADPH, coenzyme F420, component B (a new cofactor of unknown structure), ATP, Mg2+, and methyl coenzyme M.
甲烷杆菌的甲基辅酶M甲基还原酶系统已实现了重大简化。500,000道尔顿的氢化酶复合物已被NADPH - 辅酶F420氧化还原酶所取代。通过使用这种电子生成反应，发现甲基还原酶定位于组分C中，这是一种酸性蛋白质组分。在氧化还原酶和甲基还原酶存在的情况下，在氮气气氛下甲烷的形成取决于添加NADPH、辅酶F420、组分B（一种结构未知的新辅因子）、ATP、Mg2+和甲基辅酶M。
