Lo Conte G, Bartolini L, Casamenti F, Marconcini-Pepeu I, Pepeu G
Pharmacol Biochem Behav. 1982 Nov;17(5):933-7. doi: 10.1016/0091-3057(82)90475-0.
The acquisition of active (shuttle-box) and passive avoidance conditioned responses and the effects of scopolamine on acetylcholine (ACh) output in freely moving rats and on conditioned responses were investigated 20 days after placing a unilateral lesion in the magnocellular forebrain nuclei (MFN). In the lesioned rats spontaneous ACh output from the cerebral cortex ipsilateral to the lesion was slightly decreased, while on the other hand the increase in ACh output elicited by scopolamine was strongly reduced. Sham operated rats always performed more active avoidance responses than MFN lesioned rats in the daily training shuttle-box sessions, and the facilitating effect of scopolamine (1 mg/kg IP) on the shuttle-box performance was suppressed. However the lesion did not disrupt the shuttle-box performance whenever training had taken place before the lesion. In the lesioned rats retested 30 min after the training trial, an impairment of the passive avoidance response was found. The effect of the lesion was potentiated by scopolamine. The results show therefore that MFN lesions impair the cortical cholinergic mechanisms, whose activity seems to play an important role in cognitive functions.
在对大鼠的大细胞前脑核（MFN）进行单侧损伤20天后，研究了主动（穿梭箱）和被动回避条件反应的获得，以及东莨菪碱对自由活动大鼠乙酰胆碱（ACh）输出和条件反应的影响。在损伤大鼠中，损伤同侧大脑皮层的自发ACh输出略有下降，而另一方面，东莨菪碱引起的ACh输出增加则大大降低。在每日训练穿梭箱实验中，假手术大鼠总是比MFN损伤大鼠表现出更多的主动回避反应，东莨菪碱（1毫克/千克腹腔注射）对穿梭箱表现的促进作用受到抑制。然而，只要在损伤前进行过训练，损伤就不会破坏穿梭箱表现。在训练试验后30分钟重新测试的损伤大鼠中，发现被动回避反应受损。东莨菪碱增强了损伤的作用。因此，结果表明MFN损伤会损害皮层胆碱能机制，其活性似乎在认知功能中起重要作用。
