Garzon S, Bendayan M, Kurstak E
J Virol Methods. 1982 Oct;5(2):67-73. doi: 10.1016/0166-0934(82)90083-0.
HSV and DNV viral antigens have been localized by electron microscopy using the protein A-gold technique. The labelling of HSV antigens was detected over the (naked and enveloped) viral particles as well as on the cytoplasm and the nucleoplasm. In contrast, DNV antigens were revealed only over clusters of viral particles in the nucleus. The high sensitivity of the technique and the good ultrastructural preservation allowed a very fine identification of the labelled structures. Thus, the protein A-gold technique can be applied generally for the ultrastructural detection and identification of viral antigens and might be useful for diagnostic purposes.
已使用蛋白A-金技术通过电子显微镜对单纯疱疹病毒（HSV）和果蝇X病毒（DNV）的病毒抗原进行了定位。在（裸露的和有包膜的）病毒颗粒以及细胞质和核质上均检测到了HSV抗原的标记。相比之下，仅在细胞核中的病毒颗粒簇上发现了DNV抗原。该技术的高灵敏度和良好的超微结构保存使得能够非常精细地识别标记结构。因此，蛋白A-金技术可普遍应用于病毒抗原的超微结构检测和鉴定，可能对诊断有帮助。
