利用酶-金法和自身免疫血清-蛋白A-金技术对组织培养细胞核中的DNA进行超微结构定位。
Ultrastructural localization of DNA in tissue culture cell nuclei by means of enzyme-gold and autoimmune sera-protein A-gold techniques.
作者信息
Raska I, Armbruster B L, Jíra M, Hána I, Kaslík J, Rovenský J, Smetana K
出版信息
Biol Cell. 1983;48(2-3):211-4. doi: 10.1111/j.1768-322x.1984.tb00214.x.
Abstract
The post-embedding localization of DNA was investigated in cell nuclei by means of DNase I-gold and autoimmune sera-protein A-gold techniques. Using the former technique, gold particles were found mainly over euchromatin, nucleoli exhibit occasionally high labeling. In the latter technique, the use of the serum binding dsDNA confined the label mainly to condensed chromatin.
摘要
采用DNA酶I-金标法和自身免疫血清-蛋白A-金标法对细胞核中DNA进行包埋后定位研究。运用前一种技术时，发现金颗粒主要分布在常染色质上，核仁偶尔显示高标记。运用后一种技术时，结合双链DNA的血清使标记主要局限于浓缩染色质。
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