Cremer N E, Devlin V L, Riggs J L, Hagens S J
J Clin Microbiol. 1984 Sep;20(3):468-72. doi: 10.1128/jcm.20.3.468-472.1984.
Eighteen paired serum samples submitted for serodiagnosis of current infection showed anomalous antibody results by complement fixation test when tested with a battery of agents (viruses, Mycoplasma pneumoniae, and chlamydia) selected for testing on the basis of the symptoms of the patient. Seventeen serum pairs showed a fourfold or greater rise in titer of antibody to two agents in the battery, and one showed only a twofold rise in titer of antibody to the identified causative agent but an eightfold rise in titer of antibody to a heterologous agent. The 18 serum pairs were tested for IgM antibody to the two involved agents to determine whether IgM antibody tests would better distinguish the probable cause of the current infection. The serum pairs were separated into three groups based on their IgM responses. Group I consisted of six serum pairs with IgM antibody to both agents, four pairs of which showed a fourfold or greater rise in titer of IgM antibody to both agents, and two of which showed a rise in titer of IgM antibody to only one of the two agents. Group II consisted of 10 serum pairs with IgM antibody to one of the two agents, 7 pairs of which showed a fourfold or greater rise in titer of IgM antibody to the agent. Group III consisted of two serum pairs with no IgM antibody to either agent. Results show that determination of presence or absence of IgM antibody per se or demonstration of a fourfold or greater rise in specific IgM antibody titer does not always help in distinguishing the causative agent in current infections.
提交用于当前感染血清学诊断的18对血清样本，在用根据患者症状选择的一组检测试剂（病毒、肺炎支原体和衣原体）进行补体结合试验时，显示出异常的抗体结果。17对血清显示对该组中的两种试剂抗体滴度有四倍或更高的升高，一对血清仅显示对已确定的病原体抗体滴度有两倍升高，但对一种异源试剂抗体滴度有八倍升高。对这18对血清检测了针对两种相关试剂的IgM抗体，以确定IgM抗体检测是否能更好地区分当前感染的可能病因。根据IgM反应将血清对分为三组。第一组由六对血清组成，对两种试剂均有IgM抗体，其中四对显示对两种试剂的IgM抗体滴度有四倍或更高的升高，两对仅显示对两种试剂中的一种IgM抗体滴度升高。第二组由十对血清组成，对两种试剂中的一种有IgM抗体，其中七对显示对该试剂的IgM抗体滴度有四倍或更高的升高。第三组由两对血清组成，对两种试剂均无IgM抗体。结果表明，确定IgM抗体的有无或特异性IgM抗体滴度有四倍或更高的升高，并不总能有助于区分当前感染的病原体。
