Spang A E, Godowski P J, Knipe D M
J Virol. 1983 Jan;45(1):332-42. doi: 10.1128/JVI.45.1.332-342.1983.
By marker rescue with cloned herpes simplex virus 2 DNA fragments, we have mapped the temperature-sensitive mutations of a series of herpes simplex virus 2 mutants to a region of the herpes simplex virus 2 genome that lies within or near the coding sequences for the major DNA-binding protein, ICP8. In cells infected with certain of these mutants at the nonpermissive temperature, the association of the major DNA-binding protein with the cell nucleus was defective. In these cells, the DNA-binding protein accumulated in the cytoplasmic and the crude nuclear detergent wash fractions. At the permissive temperature, the maturation of the mutant ICP8 was similar to that of the wild-type viral protein. With the remainder of the mutants, the nuclear maturation of ICP8 was similar to that encoded by the wild-type virus at the nonpermissive and permissive temperatures as assayed by cell fractionation.
通过用克隆的单纯疱疹病毒2 DNA片段进行标记拯救，我们已将一系列单纯疱疹病毒2突变体的温度敏感突变定位到单纯疱疹病毒2基因组的一个区域，该区域位于主要DNA结合蛋白ICP8的编码序列内或附近。在非允许温度下感染某些这些突变体的细胞中，主要DNA结合蛋白与细胞核的结合存在缺陷。在这些细胞中，DNA结合蛋白积聚在细胞质和粗核去污剂洗涤组分中。在允许温度下，突变型ICP8的成熟与野生型病毒蛋白的成熟相似。对于其余的突变体，通过细胞分级分离测定，在非允许温度和允许温度下，ICP8的核成熟与野生型病毒编码的相似。
