Van Alstyne D, Smyrnis E M, Singh V K
Can J Microbiol. 1984 Jul;30(7):961-6. doi: 10.1139/m84-150.
We have initiated an in vitro study comparing the susceptibility of newborn and adult animals to rubella virus (RV) associated encephalitis. Glial cells from injured adult rat brain (RG cells) have been established in continuous culture and these cells were reported to restrict RV replication. When RG cells were infected, no infectious progeny virus particles were detected in tissue culture media and only five intracellular viral polypeptides could be detected using immune precipitation techniques (p75, p60, VP44, VP41, and VP19). Two polypeptides normally associated with a productive infection. VP24 and p30, could not be detected. In this report we have applied these techniques to an investigation of RV replication in newborn brain cells and have shown that relatively normal yields of all seven polypeptides found in RV-infected cells could be detected. These data indicate that some glia from newborn brain in primary culture are permissive for RV replication unlike the restricting RG cells and that this difference in restriction is critical in determining the outcome of their infection by RV.
我们已开展一项体外研究，比较新生动物和成年动物对风疹病毒（RV）相关脑炎的易感性。已建立了来自成年大鼠受伤脑的胶质细胞（RG细胞）的连续培养物，据报道这些细胞可限制RV复制。当RG细胞被感染时，在组织培养基中未检测到有感染性的子代病毒颗粒，并且使用免疫沉淀技术仅能检测到五种细胞内病毒多肽（p75、p60、VP44、VP41和VP19）。通常与生产性感染相关的两种多肽VP24和p30未被检测到。在本报告中，我们将这些技术应用于新生脑细胞中RV复制的研究，并表明在RV感染细胞中发现的所有七种多肽都能检测到相对正常的产量。这些数据表明，原代培养的新生脑的一些胶质细胞对RV复制具有容许性，这与具有限制作用的RG细胞不同，并且这种限制差异对于确定它们被RV感染的结果至关重要。
