McMurchie E J, Gibson R A, Abeywardena M Y, Charnock J S
Biochim Biophys Acta. 1983 Jan 5;727(1):163-9. doi: 10.1016/0005-2736(83)90380-2.
Diets supplemented with high levels of either saturated fatty acids or unsaturated fatty acids were fed to adult rats for a period of 9 weeks and changes in the liver mitochondrial membrane phospholipid fatty acid composition and thermal behaviour of succinate: cytochrome c reductase were determined. The dietary treatment induced a change in the omega 6 to omega 3 unsaturated fatty acid ratio in the membrane lipids, with the ratio being highest with the unsaturated fatty acid and lowest with the saturated fatty acid diet. Arrhenius plots of succinate: cytochrome c reductase activity exhibited differences in both critical temperature (Tf) and Arrhenius activation energy (Ea) depending on the type of dietary treatment. The Tf was elevated from 23 degrees C in control to 32 degrees C in the saturated fatty acid-supplemented group. No significant effect on the Tf was observed in the unsaturated fatty acid-supplemented group however higher Ea values were observed due to the unsaturated fatty acid diet. The changes in succinate: cytochrome c reductase are probably due to changes in the lipid-protein interactions in the membrane, induced by the dietary lipid supplementation.
分别用富含高水平饱和脂肪酸或不饱和脂肪酸的饲料喂养成年大鼠9周，然后测定肝脏线粒体膜磷脂脂肪酸组成的变化以及琥珀酸：细胞色素c还原酶的热行为。饮食处理导致膜脂中ω6与ω3不饱和脂肪酸比例发生变化，不饱和脂肪酸饮食组该比例最高，饱和脂肪酸饮食组最低。琥珀酸：细胞色素c还原酶活性的阿伦尼乌斯曲线显示，根据饮食处理类型的不同，临界温度（Tf）和阿伦尼乌斯活化能（Ea）均存在差异。Tf从对照组的23℃升高到饱和脂肪酸补充组的32℃。不饱和脂肪酸补充组未观察到对Tf有显著影响，但由于不饱和脂肪酸饮食，观察到较高的Ea值。琥珀酸：细胞色素c还原酶的变化可能是由于饮食中补充脂质导致膜中脂-蛋白相互作用发生改变所致。
