Gibson R A, McMurchie E J, Charnock J S, Kneebone G M
Lipids. 1984 Dec;19(12):942-51. doi: 10.1007/BF02534730.
Diets in which both the lipid content and composition (polyunsaturated to saturated fatty acid ratio) were varied were fed to rats for 20 weeks, and the effects on the tissue lipid profiles were determined. The fatty acid profile of the plasma lipids, and the phospholipid fatty acids of the mitochondrial and microsomal fractions of liver, heart, kidney and brain, as well as erythrocyte membranes were determined. Despite large differences in the level and type of lipid present in the experimental diets and in the proportion of saturated fatty acids in the plasma lipids in response to the various diets, there was little effect on the proportion of saturated to unsaturated fatty acids in the phospholipids of the various membranes examined. The major effect of altering the dietary level of polyunsaturated to saturated fatty acids was on the ratio of the omega 6/omega 3 series of unsaturated fatty acids in the membrane lipids. This change occurred in all tissues except the brain, in which only a small response to altered dietary lipid intake was observed. The omega 6/omega 3 ratio was elevated upon feeding a diet rich in omega 6 polyunsaturated fatty acids, but decreased when a diet rich in saturated fatty acids was fed. The failure to significantly alter membrane lipid saturation/unsaturation in the tissues examined would suggest that a homeostatic mechanism is operative in biological membranes and may act to buffer membranes from the effects of changes in the nature of the dietary lipid intake.
将脂质含量和组成（多不饱和脂肪酸与饱和脂肪酸的比例）均不同的饮食喂给大鼠20周，并测定其对组织脂质谱的影响。测定了血浆脂质的脂肪酸谱，以及肝脏、心脏、肾脏、大脑线粒体和微粒体部分的磷脂脂肪酸，还有红细胞膜的磷脂脂肪酸。尽管实验饮食中脂质的水平和类型以及血浆脂质中饱和脂肪酸的比例因不同饮食而存在很大差异，但对所检测的各种膜磷脂中饱和脂肪酸与不饱和脂肪酸的比例影响很小。改变饮食中多不饱和脂肪酸与饱和脂肪酸的水平的主要影响在于膜脂质中ω-6/ω-3系列不饱和脂肪酸的比例。除大脑外，所有组织均发生了这种变化，大脑中仅观察到对饮食脂质摄入量改变的微小反应。喂食富含ω-6多不饱和脂肪酸的饮食时，ω-6/ω-3比例升高，但喂食富含饱和脂肪酸的饮食时则降低。在所检测的组织中未能显著改变膜脂质的饱和/不饱和状态，这表明生物膜中存在一种稳态机制，可能起到缓冲膜免受饮食脂质摄入性质变化影响的作用。
