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一种植物tRNA基因的分离与核苷酸序列：矮牵牛天冬酰胺tRNA

Isolation and nucleotide sequence of a plant tRNA gene: petunia asparagine tRNA.

作者信息

Bawnik N, Beckmann J S, Sarid S, Daniel V

出版信息

Nucleic Acids Res. 1983 Feb 25;11(4):1117-22. doi: 10.1093/nar/11.4.1117.

DOI:10.1093/nar/11.4.1117
PMID:6298732
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC325779/
Abstract

A 14.3 kb petunia genomic DNA fragment was isolated and found to contain a single tRNA gene coding for asparagine tRNA. The nucleotide sequence of the asparagine tRNA gene and its flanking regions has been determined. This gene does not contain intervening sequences nor the 3'-end CCA sequence of the mature tRNA and presents a similar overall sequence homology (70%) to both E. coli and mammalian asparagine tRNA. As in other eukaryotic tRNA genes the 5'-flanking region does not seem to contain any special sequence that could function as a regulatory element and the 3'-end is followed by a short cluster of T that may function as the transcription termination site.

摘要

分离出一个14.3 kb的矮牵牛基因组DNA片段，发现其包含一个编码天冬酰胺tRNA的单一tRNA基因。已确定天冬酰胺tRNA基因及其侧翼区域的核苷酸序列。该基因不包含内含序列，也不包含成熟tRNA的3'-末端CCA序列，并且与大肠杆菌和哺乳动物天冬酰胺tRNA的总体序列同源性相似（70%）。与其他真核tRNA基因一样，5'-侧翼区域似乎不包含任何可作为调控元件的特殊序列，3'-末端后面是一小簇T，可能作为转录终止位点起作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9395/325779/42494224ad4e/nar00349-0214-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9395/325779/2ceee048a01b/nar00349-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9395/325779/42494224ad4e/nar00349-0214-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9395/325779/2ceee048a01b/nar00349-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9395/325779/42494224ad4e/nar00349-0214-a.jpg

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本文引用的文献

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Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA.来自非洲爪蟾DNA重复单元的编码苯丙氨酸tRNA和酪氨酸tRNA的基因的核苷酸序列。
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Nucleotide sequence of a nuclear tRNA(Gly) (GCC) gene of a higher plant, Ragi (Eleucine coracana).一种高等植物龙爪稷（Eleucine coracana）的细胞核甘氨酸tRNA（GCC）基因的核苷酸序列。
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Aspartate and asparagine tRNA genes in wheat mitochondrial DNA: a cautionary note on the isolation of tRNA genes from plants.小麦线粒体DNA中的天冬氨酸和天冬酰胺tRNA基因：关于从植物中分离tRNA基因的警示
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