Selker E, Yanofsky C
Nucleic Acids Res. 1980 Mar 11;8(5):1033-42. doi: 10.1093/nar/8.5.1033.
We have isolated and sequenced a tRNAPhe gene from Neurospora crassa. Hybridization analyses suggest that trnaPhe is the only tRNA encoded on the cloned 5 kb DNA fragment. The tRNAPhe gene contains an intervening sequence 16 nucleotides in length located one nucleotide 3' to the anticodon position. The tRNAPhe coding region of Neurospora and yeast are 91% conserved, whereas their intervening sequences are only 50% identical. The pattern of sequence conservation is consistent with a proposed secondary structure for the tRNA precursor in which the anticodon is base paired with the middle of the intervening sequence and the splice points are located in adjacent single-stranded loops. The DNA sequence following the tRNAPhe coding region is similar to sequences following other genes transcribed by RNA polymerase III in that it is AT-rich and includes a tract of A residues in the coding strand. In contrast, the sequence preceding the Neurospora tRNAPhe coding region does not resemble sequences preceding other sequenced tRNA genes.
我们从粗糙脉孢菌中分离并测序了一个苯丙氨酸转运RNA（tRNAPhe）基因。杂交分析表明，tRNAPhe是克隆的5kb DNA片段上唯一编码的转运RNA。tRNAPhe基因含有一个长度为16个核苷酸的间隔序列，位于反密码子位置下游一个核苷酸处。粗糙脉孢菌和酵母的tRNAPhe编码区有91%的保守性，而它们的间隔序列只有50%的同源性。序列保守模式与所提出的tRNA前体二级结构一致，在该结构中反密码子与间隔序列中部碱基配对，剪接位点位于相邻的单链环中。tRNAPhe编码区之后的DNA序列与RNA聚合酶III转录的其他基因之后的序列相似，因为它富含AT，并且在编码链中包含一段A残基。相比之下，粗糙脉孢菌tRNAPhe编码区之前的序列与其他已测序的tRNA基因之前的序列不同。
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