对拟南芥的三个tRNA（苯丙氨酸）核基因、一个突变基因以及一个tRNA（丙氨酸）基因进行序列分析。
Sequence analysis of three tRNA(Phe) nuclear genes and a mutated gene, and one gene for tRNA(Ala) from Arabidopsis thaliana.
作者信息
Akama K, Tanifuji S
机构信息
National Institute for Basic Biology, Okazaki, Japan.
出版信息
Plant Mol Biol. 1990 Aug;15(2):337-46. doi: 10.1007/BF00036919.
Three genes and one mutant gene for tRNA(Phe) (GAA) and one gene for tRNA(Ala) (UGC) were isolated from a whole-cell DNA library of Arabidopsis thaliana. All three tRNA(Phe) genes are identical in their nucleotide sequence, but differ in their 5' and 3' flanking regions. The mutant tRNA(Phe) (GAA) gene differs from the other three genes by one nucleotide change from highly conserved G to C at the 57th nucleotide position. The primary structure of the first tRNA(Ala) gene was also determined in this experiment.
从拟南芥全细胞DNA文库中分离出了3个tRNA（Phe）（GAA）基因、1个tRNA（Phe）（GAA）突变基因和1个tRNA（Ala）（UGC）基因。所有3个tRNA（Phe）基因的核苷酸序列相同，但5'和3'侧翼区域不同。突变的tRNA（Phe）（GAA）基因与其他3个基因的区别在于，在第57个核苷酸位置有一个从高度保守的G到C的单核苷酸变化。本实验还确定了第一个tRNA（Ala）基因的一级结构。
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