克隆的鸡α-珠蛋白cDNA的完整核苷酸序列
Complete nucleotide sequence of a cloned chicken alpha-globin cDNA.
作者信息
Deacon N J, Shine J, Naora H
出版信息
Nucleic Acids Res. 1980 Mar 25;8(6):1187-99. doi: 10.1093/nar/8.6.1187.
Chicken globin double-stranded cDNA was synthesised from anaemic adult reticulocyte alpha- and beta-globin mRNA and ligated into the Hind III site of pBR322 using synthetic Hind III decamers. Transformation of E. coli x1776 resulted in the production of a number of alpha- and beta-cDNA clones. One of the alpha-type clones (pCG alpha-8) was fully sequenced and found to code for neither alpha A- nor alpha D-globin. Partial sequencing of the other alpha-cDNA clones indicates that they are all of the same type.
鸡珠蛋白双链cDNA是由成年贫血网织红细胞的α-和β-珠蛋白mRNA合成的，并使用合成的Hind III十聚体连接到pBR322的Hind III位点。将其转化大肠杆菌x1776后产生了许多α-和β-cDNA克隆。其中一个α型克隆（pCGα-8）进行了全序列测定，发现它既不编码αA-珠蛋白也不编码αD-珠蛋白。对其他α-cDNA克隆的部分测序表明它们都是同一类型。
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