Cutlip R C, Lehmkuhl H D
Am J Vet Res. 1982 Dec;43(12):2101-7.
Pulmonary changes in five 1-week-old, colostrum-deprived lambs transtracheally inoculated with parainfluenza type 3 virus were studied by immunofluorescent, microscopic, and ultrastructural techniques. The lambs were killed at postinoculation days (PID) 3, 5, and 7. Immunofluorescence specific for parainfluenza type 3 virus was first seen in small airways and alveolar epithelium and later in the lumens of airways and alveoli and, to a lesser extent, in the interstitium of the lungs. Grossly, there were multifocal areas of consolidation in all lobes of the lungs. These areas were characterized microscopically by bronchiolitis and interstitial pneumonitis. The bronchiolitis involved the terminal airways and consisted of necrosis and sloughing of epithelial cells followed by hyperplasia of the epithelium. The interstitial lesion comprised extensive infiltration of alveolar septa and alveoli with macrophages and the necrosis of alveolar epithelium. This was followed by hyperplasia of the epithelium. Degenerated bronchiolar and alveolar epithelium contained numerous intracytoplasmic inclusions early in the infection, but such inclusions were not seen in the lambs killed at PID 7. The degenerated changes were also seen with the electron microscope, as were numerous inclusions of viral nucleoprotein and a few viral buds at PID 3 and 5. Viral inclusions and buds were seen in ciliated and nonciliated bronchial epithelial cells and type I and type II alveolar epithelial cells.
采用免疫荧光、显微镜及超微结构技术，对5只1周龄、未摄入初乳且经气管接种3型副流感病毒的羔羊的肺部变化进行了研究。这些羔羊在接种后第3、5和7天被处死。3型副流感病毒特异性免疫荧光最初见于小气道和肺泡上皮，随后见于气道和肺泡腔，在肺间质中也有少量出现。大体上，肺的所有叶均有多处实变区域。显微镜下，这些区域的特征为细支气管炎和间质性肺炎。细支气管炎累及终末气道，表现为上皮细胞坏死和脱落，随后上皮增生。间质性病变包括肺泡间隔和肺泡内巨噬细胞广泛浸润以及肺泡上皮坏死，随后上皮增生。在感染早期，变性的细支气管和肺泡上皮细胞内含有大量胞质内包涵体，但在接种后第7天处死的羔羊中未见此类包涵体。在电子显微镜下也可见到变性变化，在接种后第3天和第5天还可见到大量病毒核蛋白包涵体和少量病毒芽。在纤毛和非纤毛支气管上皮细胞以及I型和II型肺泡上皮细胞中可见病毒包涵体和芽。
