Mode of chromatin reconstitution. Elements controlling globin gene transcription.

The mode of reconstitution has been determined for the factors that control globin gene transcription in chicken reticulocyte chromatin. During chromatin reconstitution, elements in the nonhistone protein fraction that control the globin gene reassociate with the DNA before the histones. From our results, it appears that it is necessary for these elements to reassociate with the DNA before the histones for the globin gene to be transcribed.