Hendrick D, Tolstoshev P, Randlett D
Gene. 1977;2(3-4):147-58. doi: 10.1016/0378-1119(77)90014-2.
A nuclease-sensitive fraction was obtained from chick reticulocyte chromatin by brief digestion with an endonuclease (DNAase II, deoxyribonucleate 3'-oligonucleotidohydrolase, EC 3.1.4.6). The nuclease-sensitive fraction typically contained less than 1% of the chromatin-DNA but about 50% or more of the nascent chromatin-bound RNA. Hybridization of chick globin complementary DNA to the DNA component of the nuclease-sensitive fraction of reticulocyte chromatin indicated a 3--5 fold enrichment for the globin coding region of the chromatin. The control experiment utilizing DNA from a nuclease-sensitive fraction of chick liver chromatin did not show a comparable enrichment for the globin coding region. This suggests that the endonuclease-effected enrichment for the globin coding region in the nuclease-sensitive fraction of reticulocyte chromatin is to some degree specific for structural genes transcribed in reticulocytes.
通过用一种核酸内切酶（DNA酶II，脱氧核糖核酸3'-寡核苷酸水解酶，EC 3.1.4.6）短暂消化，从鸡网织红细胞染色质中获得了一个核酸酶敏感组分。该核酸酶敏感组分通常包含不到1%的染色质DNA，但约50%或更多的新生染色质结合RNA。将鸡珠蛋白互补DNA与网织红细胞染色质的核酸酶敏感组分的DNA成分杂交，结果表明染色质的珠蛋白编码区域富集了3至5倍。利用来自鸡肝染色质核酸酶敏感组分的DNA进行的对照实验，并未显示出珠蛋白编码区域有类似的富集。这表明，核酸酶对网织红细胞染色质核酸酶敏感组分中珠蛋白编码区域的富集在某种程度上对网织红细胞中转录的结构基因具有特异性。
