Johnson D C, Spear P G
Cell. 1983 Mar;32(3):987-97. doi: 10.1016/0092-8674(83)90083-1.
The O-linked oligosaccharides on mature forms of herpes simplex virus type 1 (HSV1) glycoproteins were characterized, and were found to account largely for the lower electrophoretic mobilities of these forms relative to the mobilities of immature forms. Other posttranslational modifications of HSV1 glycoproteins (designated gB, gC, gD and gE) were related temporally to the discrete shifts in electrophoretic mobilities that signal acquisition of the O-linked oligosaccharides. Fatty acid acylation (principally of gE) could be detected just prior to the shifts, whereas conversion of high-mannose-type N-linked oligosaccharides to the complex type occurred coincident with the shifts. The addition of O-linked oligosaccharides did not occur in cells treated with the ionophore monensin or in a ricin-resistant cell line defective in the processing of N-linked oligosaccharides. We conclude that extension of O-linked oligosaccharide chains on HSV1 glycoproteins, and probably also attachment of the first O-linked sugars, occurs as a late posttranslational modification in the Golgi apparatus.
对1型单纯疱疹病毒（HSV1）糖蛋白成熟形式上的O-连接寡糖进行了表征，发现这些形式相对于未成熟形式电泳迁移率较低，主要是由O-连接寡糖造成的。HSV1糖蛋白（命名为gB、gC、gD和gE）的其他翻译后修饰在时间上与电泳迁移率的离散变化相关，这些变化标志着O-连接寡糖的获得。在迁移率变化之前可以检测到脂肪酸酰化（主要是gE的），而高甘露糖型N-连接寡糖向复合型的转化与迁移率变化同时发生。在用离子载体莫能菌素处理的细胞中，或在N-连接寡糖加工有缺陷的蓖麻毒素抗性细胞系中，未发生O-连接寡糖的添加。我们得出结论，HSV1糖蛋白上O-连接寡糖链的延伸，可能还有第一个O-连接糖的连接，是在高尔基体中发生的晚期翻译后修饰。
