Sowers J R, Beck F, Stern N, Raghavan S R
Clin Exp Hypertens A. 1983;5(1):71-86. doi: 10.3109/10641968309048811.
Ouabain-sensitive Na+-K+-ATPase activity in red cell membranes, kidney cortical tissue, myocardium and adrenal glomerulosa tissue was examined in SHR and WKY rats at 6, 9, and 12 weeks of age. Red cell membrane enzyme activity was decreased (p less than 0.001) at 9 and 12 weeks of age in SHR. This activity was negatively correlated (r = -0.69, p less than .005) with blood pressure at 9 and 12 weeks. Kidney cortical enzyme activity was also decreased (p less than 0.001) in the SHR at 9 and 12 weeks of age. This decreased kidney enzyme activity was also inversely related to 9 and 12 week blood pressures (r = -0.71, p less than 0.001), urinary Na excretion (r = -0.62, p less than .005), and urinary Ca and K excretion. Myocardial enzyme activity was not decreased until 12 weeks in the SHR, and adrenal glomerulosa activity was not different in the SHR and WKY at any of the three ages that this enzyme was measured. Of the tissues examined decreased Na+-K+-ATPase activity in the erythrocyte membrane and in kidney cortical tissue appears to coincide best with the development of hypertension in the SHR. This study lends further support to the concept that alterations in membrane cation transport may be an important factor in the development of high blood pressure in SHR.
在6周、9周和12周龄的自发性高血压大鼠（SHR）和WKY大鼠中，检测了红细胞膜、肾皮质组织、心肌和肾上腺球状带组织中哇巴因敏感的钠钾ATP酶活性。SHR在9周和12周龄时红细胞膜酶活性降低（p<0.001）。该活性在9周和12周时与血压呈负相关（r = -0.69，p<0.005）。SHR在9周和12周龄时肾皮质酶活性也降低（p<0.001）。这种肾酶活性的降低也与9周和12周时的血压（r = -0.71，p<0.001）、尿钠排泄（r = -0.62，p<0.005）以及尿钙和钾排泄呈负相关。SHR直到12周时心肌酶活性才降低，在检测该酶的三个年龄阶段中的任何一个阶段，SHR和WKY的肾上腺球状带活性均无差异。在所检测的组织中，红细胞膜和肾皮质组织中钠钾ATP酶活性的降低似乎与SHR高血压的发展最为吻合。这项研究进一步支持了膜阳离子转运改变可能是SHR高血压发展的一个重要因素这一概念。
