Myhre E B, Kuusela P
Infect Immun. 1983 Apr;40(1):29-34. doi: 10.1128/iai.40.1.29-34.1983.
A total of 387 bacterial strains belonging to 35 species were tested in direct binding experiments for the uptake of purified radiolabeled human fibronectin. Positive binding was found in group A, C, and G streptococci and in Staphylococcus aureus. The group C streptococcal species Streptococcus equisimilis, Streptococcus zooepidemicus, Streptococcus equi, and Streptococcus dysgalactiae were uniformly reactive with fibronectin. Beta-hemolytic bovine group G streptococci showed the same degree of reactivity as human group G strains. In contrast, only 4 out of 15 alpha-hemolytic bovine group G strains were able to bind fibronectin. The uptake of fibronectin measured at room temperature with a human group G streptococcus was a slow, time-dependent process with maximum binding after approximately 1 h. Human polyclonal immunoglobulin G and serum albumin tested in inhibition experiments did not affect the fibronectin binding. Fibronectin seems, therefore, to interact with a surface component that is different from the specific binding sites previously described for human immunoglobulin G and serum albumin.
在直接结合实验中，对属于35个物种的总共387株细菌菌株进行了检测，以观察其对纯化的放射性标记人纤连蛋白的摄取情况。在A组、C组和G组链球菌以及金黄色葡萄球菌中发现了阳性结合。C组链球菌中的马链球菌兽疫亚种、马链球菌马亚种、马链球菌和停乳链球菌均能与纤连蛋白发生一致的反应。β溶血牛G组链球菌与人G组菌株表现出相同程度的反应性。相比之下，15株α溶血牛G组菌株中只有4株能够结合纤连蛋白。用人G组链球菌在室温下测量的纤连蛋白摄取是一个缓慢的、时间依赖性的过程，大约1小时后结合达到最大值。在抑制实验中测试的人多克隆免疫球蛋白G和血清白蛋白不影响纤连蛋白的结合。因此，纤连蛋白似乎与一种表面成分相互作用，该表面成分不同于先前描述的与人免疫球蛋白G和血清白蛋白的特异性结合位点。
