Allen G P, Yeargan M R, Bryans J T
Infect Immun. 1983 Apr;40(1):436-9. doi: 10.1128/iai.40.1.436-439.1983.
The effect of in vitro and in vivo serial virus passage on the genetic stability of equine herpesvirus 1 (EHV-1) was investigated by restriction endonuclease analysis of the viral DNA. DNAs of EHV-1 isolates at different passage levels in cultured cells or in Syrian hamsters were compared by electrophoresis of the DNA cleavage fragments produced by restriction endonuclease digestion. No changes were observed in the restriction profile of the DNAs of EHV-1 strains after 100 sequential passages in cultured equine cells. However, serial passage of the virus in hamsters or in cells of non-equine origin quickly gave rise to alterations in the viral DNA. These changes occurring in the restriction endonuclease profiles of EHV-1 DNA during serial virus passage in non-equine cells or animals hosts could be explained by sequence additions or deletions to preexisting restriction fragments resulting in variation in their electrophoretic mobilities.
通过对病毒DNA进行限制性内切酶分析，研究了体外和体内连续病毒传代对马疱疹病毒1型（EHV-1）遗传稳定性的影响。通过对限制性内切酶消化产生的DNA切割片段进行电泳，比较了EHV-1分离株在培养细胞或叙利亚仓鼠中不同传代水平的DNA。在马培养细胞中连续传代100次后，EHV-1毒株的DNA限制性图谱未观察到变化。然而，该病毒在仓鼠或非马源细胞中连续传代很快导致病毒DNA发生改变。在非马细胞或动物宿主中连续病毒传代期间，EHV-1 DNA限制性内切酶图谱中出现的这些变化可以通过在现有限制性片段中添加或缺失序列来解释，这导致其电泳迁移率发生变化。
