Hagiwara A, Yoneyama T, Hashimoto I
J Gen Virol. 1983 Feb;64 (Pt 2):499-502. doi: 10.1099/0022-1317-64-2-499.
Small- and large-plaque-producing viruses were selected from the enterovirus 71 (E71) prototype, BrCr. The small-plaque virus was a temperature-resistant (tr) strain which could multiply at 39.5 degrees C as well as 35 degrees C. The large-plaque virus was temperature-sensitive (ts) and could not grow at 39.5 degrees C. It was shown that the ts strain was much less neurovirulent for monkeys than the tr strain. Both tr and ts strains reacted with homologous and heterologous antibodies in cross-neutralization tests. While no difference was found between tr and ts strains in the size of virion polypeptides, the VP1 polypeptide could be differentiated by isoelectric focusing. Genomic differences between the two strains were found by oligonucleotide mapping.
从小儿麻痹症病毒71型（E71）原型BrCr中筛选出产生小噬斑和大噬斑的病毒。小噬斑病毒是一种耐温（tr）毒株，能在39.5℃和35℃下增殖。大噬斑病毒对温度敏感（ts），在39.5℃下不能生长。结果表明，ts毒株对猴子的神经毒性远低于tr毒株。在交叉中和试验中，tr和ts毒株均能与同源和异源抗体发生反应。虽然tr和ts毒株在病毒粒子多肽大小上没有差异，但VP1多肽可通过等电聚焦进行区分。通过寡核苷酸图谱分析发现了两株病毒之间的基因组差异。
