Sarma P S, Rowe W P
J Virol. 1983 Mar;45(3):956-60. doi: 10.1128/JVI.45.3.956-960.1983.
The addition of guinea pig complement was found to enhance the neutralizing capacity of mouse antibodies directed against the endogenous ecotropic murine leukemia viruses. The same immune sera, when tested without complement, had low or negligible neutralizing capacities, regardless of whether freshly harvested, unfrozen virus was used to preserve virus infectivity. Antibodies in high titers were found in sera from NFS congenic mice carrying the mouse leukemia virus inducing locus Akv-2. These mouse antibodies were type specific and failed to neutralize either Friend or Moloney leukemia virus. The mouse serum immunoglobulin fraction containing the complement-dependent antibodies was tentatively identified as immunoglobulin M.
发现添加豚鼠补体可增强小鼠抗体针对内源性嗜亲性鼠白血病病毒的中和能力。相同的免疫血清，在不添加补体进行检测时，无论是否使用新鲜收获、未冷冻的病毒来保持病毒感染性，其具有的中和能力都很低或可忽略不计。在携带诱导小鼠白血病病毒的基因座Akv - 2的NFS同源小鼠血清中发现了高滴度抗体。这些小鼠抗体具有型特异性，无法中和弗氏或莫洛尼白血病病毒。初步确定含有补体依赖性抗体的小鼠血清免疫球蛋白部分为免疫球蛋白M。
