揭示噬菌体T4 DNA复制所需解旋活性的细菌和噬菌体突变。
Bacterial and phage mutations that reveal helix-unwinding activities required for bacteriophage T4 DNA replication.
作者信息
Gauss P, Doherty D H, Gold L
出版信息
Proc Natl Acad Sci U S A. 1983 Mar;80(6):1669-73. doi: 10.1073/pnas.80.6.1669.
Abstract
An Escherichia coli strain with a mutation in the optA gene restricts the growth of bacteriophage T4 strains partially defective in gene 43 (DNA polymerase) or missing gene dda (DNA-dependent ATPase). The mutations in the dda gene inactivate a DNA-dependent ATPase that has been shown to have DNA helicase activity in vitro. We show that the restriction of phage growth after infection of the optA bacterium is the result of a block in DNA replication. We infer that the block arises from a defect in DNA unwinding.
摘要
一株在optA基因发生突变的大肠杆菌菌株会限制基因43（DNA聚合酶）部分缺陷或缺失基因dda（DNA依赖性ATP酶）的噬菌体T4菌株的生长。dda基因中的突变会使一种DNA依赖性ATP酶失活，该酶在体外已被证明具有DNA解旋酶活性。我们表明，感染optA细菌后噬菌体生长受限是DNA复制受阻的结果。我们推断该阻滞是由DNA解旋缺陷引起的。
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