通过与克隆基因杂交揭示的噬菌体T4 DNA复制的起源
Origins of phage T4 DNA replication as revealed by hybridization to cloned genes.
作者信息
Halpern M E, Mattson T, Kozinski A W
出版信息
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6137-41. doi: 10.1073/pnas.76.12.6137.
Abstract
[3H]Thymidine-labeled progeny DNA was isolated after infection of Escherichia coli with two different bacteriophage T4 mutants. These strands were isolated shortly after the initiation of DNA replication and hybridized to 15 different (EcoRI) T4 restriction fragments cloned in plasmid vectors. Uniformly labeled T4 [32P]DNA extracted from phage particles was cohybridized as a normalizing reference. The results obtained lead to the conclusions that, among the loci tested, initiation occurs predominantly in the area of genes 50-5 and less prominently in the area of genes 25-29. However, our data do not support the idea of initiation in the area of genes 40-43. In contrast, this area displays the least replication among the genes tested.
摘要
用两种不同的噬菌体T4突变体感染大肠杆菌后，分离出[3H]胸苷标记的子代DNA。这些链在DNA复制开始后不久被分离出来，并与克隆在质粒载体中的15个不同的（EcoRI）T4限制片段杂交。从噬菌体颗粒中提取的均匀标记的T4 [32P]DNA作为标准化参考进行共杂交。所得结果得出以下结论：在所测试的基因座中，起始主要发生在基因50 - 5区域，而在基因25 - 29区域则不太明显。然而，我们的数据不支持在基因40 - 43区域起始的观点。相反，在所测试的基因中，该区域的复制最少。
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