Icard-Liepkalns C, Liepkalns V A, Webb T E
Biochem Biophys Res Commun. 1983 Jan 14;110(1):96-102. doi: 10.1016/0006-291x(83)91265-2.
Using labelled single copy DNA to cytoplasmic messenger RNA from a glioma cell line, it is shown by the excess RNA hybridization technique that a human glioma and a human fetal brain cell line both contain the mid and low abundancy classes of cytoplasmic messenger RNA. However, the high abundancy class present in the glioma cells is absent from the hybridization profile of the fetal cell line. Most of the nuclear RNA species complementary to this single copy DNA were present in the low abundancy class of both cell types; the mid-abundancy class was present in much lower concentration than in glioma cytoplasmic RNA and the high abundancy class was essentially absent. The extent of formation of S1-nuclease resistant hybrids indicated that some of the messengers which are present in the high abundancy class in the cytoplasm of glioma cells are present in the lower abundancy classes of fetal brain cells. Thus the glioma cells appear to exhibit a higher degree of specialization potential than the embryonic cells.
使用标记的单拷贝DNA与神经胶质瘤细胞系的细胞质信使RNA进行杂交，通过过量RNA杂交技术表明，人神经胶质瘤和人胎儿脑细胞系都含有中等丰度和低丰度类别的细胞质信使RNA。然而，胎儿细胞系的杂交图谱中不存在神经胶质瘤细胞中存在的高丰度类别。与这种单拷贝DNA互补的大多数核RNA种类在两种细胞类型的低丰度类别中都有；中等丰度类别存在的浓度比神经胶质瘤细胞质RNA中的低得多，高丰度类别基本不存在。S1核酸酶抗性杂交体的形成程度表明，在神经胶质瘤细胞质中高丰度类别中存在的一些信使在胎儿脑细胞的较低丰度类别中也存在。因此，神经胶质瘤细胞似乎比胚胎细胞表现出更高程度的特化潜力。
