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转染入仓鼠成纤维细胞后载体DNA的聚合作用。

Polymerization of vector DNA after transfection into hamster fibroblast cells.

作者信息

Wang M L, Lee A S

出版信息

Biochem Biophys Res Commun. 1983 Jan 27;110(2):593-601. doi: 10.1016/0006-291x(83)91191-9.

DOI:10.1016/0006-291x(83)91191-9
PMID:6301457
Abstract

We have transformed hamsters lung fibroblasts with two cloned, selectable markers, the widely used pSV2gpt vector and pNEO3, which confers resistance to neomycin. Analysis of DNA extracted from both individual and pooled groups of several hundred transformants reveal that in these carrier DNA-free transformation experiments, the vector DNA are found in concatenate forms in the transformants. The copy number of the integrated vectors can vary from a few to several hundred. In addition, the efficiency of transformation is ten times higher in the case of pSV2gpt.

摘要

我们用两个克隆的、可选择的标记物，即广泛使用的pSV2gpt载体和赋予对新霉素抗性的pNEO3，对仓鼠肺成纤维细胞进行了转化。对从数百个转化体的单个和混合组中提取的DNA进行分析发现，在这些无载体DNA的转化实验中，载体DNA以串联形式存在于转化体中。整合载体的拷贝数可以从几个到几百个不等。此外，pSV2gpt的转化效率要高十倍。

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