Lindena J, Sommerfeld U, Höpfel C, Wolkersdorfer R, Trautschold I
Enzyme. 1983;29(2):100-8. doi: 10.1159/000469614.
From human and dog peripheral blood, thrombocytes (TRC), mononuclear cells (MNC), polymorphonuclear cells (PMNC), and red blood cells (RBC), were harvested after separation on a discontinuous Percoll gradient in a two-step centrifugation procedure, and 12 enzyme activities were determined in these highly purified cells. The enzyme activities measured are generally severalfold higher than previously reported, a fact which is attributed to the gentle and time-shortening isolation in Percoll, the cell disruption technique using detergent and the enzyme test conditions.
从人和狗的外周血中，通过两步离心法在不连续的Percoll梯度上分离后收获血小板（TRC）、单核细胞（MNC）、多形核细胞（PMNC）和红细胞（RBC），并在这些高度纯化的细胞中测定了12种酶活性。所测得的酶活性通常比先前报道的高几倍，这一事实归因于在Percoll中温和且缩短时间的分离、使用去污剂的细胞破碎技术以及酶测试条件。
