芽孢杆菌属中的种间转化：异源基因间转化的机制
Interspecies transformation in Bacillus: mechanism of heterologous intergenote transformation.
作者信息
Harris-Warrick R M, Lederberg J
出版信息
J Bacteriol. 1978 Mar;133(3):1246-53. doi: 10.1128/jb.133.3.1246-1253.1978.
Abstract
Bacillus subtilis-Bacillus globigii hybrids were made by integration of the B. globigii aromatic region (aroB to aroE) as an intergenote in the B. subtillis chromosome. Transformation of the heterologous intergenote by B. subtillis DNA (or vice versa) occurred at about 10% of the frequency of homologous transformation by hybrid donors into the same region. Heterologous intergenote crosses were unusually sensitive to shear fragmentations of donor DNA to sizes less than 30 X 10(6) to 40 X 10(6) daltons. In all cases, the entire intergenote was transferred en bloc. Homologous transformation of intergenote markers by B. globigii DNA was not unusually shear sensitive, and linkage was normal for markers in the intergenote. A model is proposed in which efficient heterologous intergenote transformation occurs by recognition and base pairing of homologous DNA sequences of both flanks of the intergenote.
摘要
枯草芽孢杆菌-球形芽孢杆菌杂种是通过将球形芽孢杆菌的芳香族区域（aroB至aroE）作为基因间区整合到枯草芽孢杆菌染色体中而构建的。枯草芽孢杆菌DNA对异源基因间区的转化（反之亦然）发生频率约为杂种供体对同一区域同源转化频率的10%。异源基因间区杂交对供体DNA剪切成小于30×10⁶至40×10⁶道尔顿大小的片段异常敏感。在所有情况下，整个基因间区都是作为一个整体转移的。球形芽孢杆菌DNA对基因间区标记的同源转化对剪切不异常敏感，并且基因间区标记的连锁是正常的。提出了一个模型，其中有效的异源基因间区转化是通过基因间区两侧同源DNA序列的识别和碱基配对发生的。
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本文引用的文献
1
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Genetics. 1963 Apr;48(4):529-51. doi: 10.1093/genetics/48.4.529.
2
Transformation between Haemophilus influenzae and Haemophilus parainfluenzae.流感嗜血杆菌与副流感嗜血杆菌之间的转化
J Bacteriol. 1970 Oct;104(1):390-400. doi: 10.1128/jb.104.1.390-400.1970.
3
Transformation and transduction of a large deletion mutation in Bacillus subtilis.枯草芽孢杆菌中一个大缺失突变的转化与转导
Mol Gen Genet. 1972;118(4):311-20. doi: 10.1007/BF00333566.
4
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5
Homology in capsular transformation reactions in Pneumococcus.肺炎球菌荚膜转化反应中的同源性。
Mol Gen Genet. 1972;116(1):68-83. doi: 10.1007/BF00334261.
6
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Mol Gen Genet. 1971;110(1):1-22. doi: 10.1007/BF00276040.
7
Heterospecific transformation of pneumococcus and streptococcus. 3. Reduction of linkage.肺炎球菌和链球菌的异种特异性转化。3. 连锁的减少。
Genetics. 1967 Dec;57(4):851-64. doi: 10.1093/genetics/57.4.851.
8
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10
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