Hansson E, Rönnbäck L
Neurochem Res. 1983 Mar;8(3):375-88. doi: 10.1007/BF00965727.
Protein metabolism was studied in astroglial primary cultures, grown for different time periods. Removal of fetal calf-serum for two days led to a morphological differentiation consisting of retraction of cell soma and extention of processes. There was a prominent decrease in total soluble protein and a decrease in [3H]valine incorporation into soluble protein. Dibutyrylcyclic-3'-5'-adenosine monophosphate (dB-cAMP)-treatment for two days also changed morphology in a similar way, but had no effect on [3H]valine incorporation into protein. After addition of soluble brain extract to the cultures an increased [3H]valine incorporation into soluble protein was seen together with a morphological differentiation, more pronounced in the presence than in the absence of fetal calf-serum. Proteins were secreted from the cells into the incubation medium and studied by electrophoresis. The more prominent protein bands had m.w. in the region of 10,000-100,000 daltons. The amount of newly synthesized proteins released into the medium was unchanged (or decreased slightly in 14 and 16 day old cultures) after addition of dB-cAMP" or soluble brain extract, and was much reduced after removal of fetal calf-serum.
在不同时间段培养的星形胶质细胞原代培养物中研究了蛋白质代谢。去除胎牛血清两天导致了一种形态分化，包括细胞体收缩和突起延伸。总可溶性蛋白显著减少，并且[³H]缬氨酸掺入可溶性蛋白的量也减少。用二丁酰环磷腺苷（dB-cAMP）处理两天也以类似方式改变了形态，但对[³H]缬氨酸掺入蛋白质没有影响。向培养物中添加可溶性脑提取物后，可见[³H]缬氨酸掺入可溶性蛋白增加，同时伴有形态分化，在有胎牛血清存在时比不存在时更明显。蛋白质从细胞分泌到培养液中，并通过电泳进行研究。更明显的蛋白条带分子量在10,000 - 100,000道尔顿范围内。添加dB-cAMP或可溶性脑提取物后，释放到培养液中的新合成蛋白量没有变化（或在14天和16天龄的培养物中略有减少），而去除胎牛血清后则大幅减少。
