Hansson E, Rönnbäck L, Sellström A
J Neurochem. 1981 Apr;36(4):1472-5. doi: 10.1111/j.1471-4159.1981.tb00588.x.
This paper reports on the electrophoretic protein/polypeptide pattern of a rat brain primary culture. For comparison, the polypeptide pattern of neuronal and glial enriched fractions from adult rat brain and cerebral hemispheres from newborn and adult rat have been analysed. Water-soluble and SDS-extractable polypeptide fractions appeared and/or increased in amount in the cultures until confluency. The polypeptide pattern of the cultures most resembled that of the glial cell fractions, showing some of this fraction's specificity. Removal of fetal calf serum and addition of 0.1 mM-dibutyryl cyclic adenosine monophosphate (dB-cAMP) produced few changes in the electrophoretic pattern. The study thus provides evidence in favour of the astroglial nature of the brain primary culture. It also shows that the cells undergo some maturation in the culture.
本文报道了大鼠脑原代培养物的蛋白质/多肽电泳图谱。为作比较，分析了成年大鼠脑神经元和胶质细胞富集组分以及新生和成年大鼠大脑半球的多肽图谱。在培养物达到汇合之前，水溶性和SDS可提取的多肽组分出现和/或数量增加。培养物的多肽图谱与胶质细胞组分的图谱最为相似，显示出该组分的一些特异性。去除胎牛血清并添加0.1 mM二丁酰环磷酸腺苷（dB-cAMP），电泳图谱几乎没有变化。因此，该研究为脑原代培养物的星形胶质细胞性质提供了证据。它还表明细胞在培养过程中经历了一些成熟过程。
