de Jong J C, Wigand R, Kidd A H, Wadell G, Kapsenberg J G, Muzerie C J, Wermenbol A G, Firtzlaff R G
J Med Virol. 1983;11(3):215-31. doi: 10.1002/jmv.1890110305.
About 200 antigenically related adenoviruses were isolated from cases of infantile diarrhoea in the Netherlands and North-West Germany. The viruses were fastidious and failed to replicate serially in human diploid fibroblasts and in primary human embryonic kidney cells. A number of strains were established in HeLa, HEp-2, Graham (293), cynomolgus monkey kidney, and Chang conjunctival cells. The viruses were mammalian adenoviruses by the usual criteria. No relationship to the 39 known human adenovirus species was found, either by neutralization tests or by haemagglutination inhibition tests. Neutralization tests showed two distinct variants, represented by strains Tak and Dugan. The variants were identical in haemagglutination inhibition tests. DNA restriction enzyme analysis showed Tak and Dugan to have considerably different genomes, indicating that these variants should be classified as different species (Wadell et al, 1983). It is proposed that the variants should be called Mastadenovirus h 40 (with reference strains Dugan and Hovi X) and Mastadenovirus h 41 (with reference strain Tak). Neutralization and haemagglutination inhibition tests demonstrated that the viruses from Glasgow and Helsinki (Hovi X) described by Johansson et al [1980] and by Kidd and Madeley [1981] belong to these two adenovirus species.
在荷兰和德国西北部，从婴儿腹泻病例中分离出了约200种抗原相关的腺病毒。这些病毒苛求，无法在人二倍体成纤维细胞和原代人胚胎肾细胞中连续传代。一些毒株在HeLa、HEp-2、Graham（293）、食蟹猴肾细胞和张氏结膜细胞中得以建立。按照通常标准，这些病毒属于哺乳动物腺病毒。通过中和试验或血凝抑制试验，均未发现它们与已知的39种人类腺病毒种类有任何关系。中和试验显示出两种不同的变体，以Tak和Dugan毒株为代表。在血凝抑制试验中，这些变体是相同的。DNA限制性内切酶分析表明，Tak和Dugan具有相当不同的基因组，这表明这些变体应被归类为不同的种类（瓦德尔等人，1983年）。建议将这些变体分别称为人腺病毒h 40（参考毒株为Dugan和Hovi X）和人腺病毒h 41（参考毒株为Tak）。中和试验和血凝抑制试验表明，约翰松等人[1980年]以及基德和马德利[1981年]描述的来自格拉斯哥和赫尔辛基的病毒（Hovi X）属于这两种腺病毒种类。
