Wigand R, Baumeister H G, Maass G, Kühn J, Hammer H J
J Med Virol. 1983;11(3):233-40. doi: 10.1002/jmv.1890110306.
Thirty-four out of 64 faecal samples with adenovirus particles, as seen by electron microscopy, were found to contain adenovirus 40 or 41 by direct isolation and neutralization in Chang's conjunctival cells, mostly within one week. (Ad40 and 41 candidate viruses are serologically related.) 6 other adenovirus species were isolated; 6 samples gave equivocal results, and 18 were negative. A genus-specific ELISA with an antihexon coat yielded positive results in 40 out of 55 samples; the test failed to identify adenovirus antigen in 10 out of 17 specimens, which were found negative by culture. All of them were negative by immunfluorescence of inoculated Chang cell cultures. Hence the failures are probably due to insufficient amount of virus in the samples. The predominance of only two adenovirus species associated with gastroenteritis in children and the ease of cultivating and identifying them should help to elucidate their etiological significance.
在64份经电子显微镜观察发现含有腺病毒颗粒的粪便样本中，通过在张氏结膜细胞中直接分离和中和试验，发现其中34份样本含有腺病毒40型或41型，大多在一周内检出。（腺病毒40型和41型候选病毒在血清学上相关。）还分离出了其他6种腺病毒；6份样本结果不明确，18份为阴性。用抗六邻体衣壳的属特异性酶联免疫吸附测定（ELISA）在55份样本中的40份得到阳性结果；该检测在17份标本中的10份未能鉴定出腺病毒抗原，这些标本经培养为阴性。对接种的张氏细胞培养物进行免疫荧光检测，所有这些标本均为阴性。因此，检测失败可能是由于样本中病毒量不足。仅两种与儿童肠胃炎相关的腺病毒种类占优势，以及它们易于培养和鉴定，这应有助于阐明它们的病因学意义。
