Kidd A H, Cosgrove B P, Brown R A, Madeley C R
J Hyg (Lond). 1982 Jun;88(3):463-74. doi: 10.1017/s0022172400070327.
Attempts were made to isolate viruses from babies' stools that contained adenoviruses detected by electron microscopy. One hundred and fifty-nine specimens from 71 children were studied and adenoviruses of established serotypes were isolated from 81 stools. Serial stool samples containing adenovirus particles were obtained from 35 children, and prolonged shedding of recognized serotypes was common. Simultaneous and sequential infections by different serotypes were also observed. Thirty-six children shed adenoviruses that could not be isolated using cell cultures normally used to detect adenoviruses, and nine of these children also shed adenoviruses of established serotypes. Passage in Chang conjunctival cell culture allowed characterization of fastidious adenoviruses from 14 children as members of a previously unrecognized serotype.
研究人员尝试从通过电子显微镜检测出含有腺病毒的婴儿粪便中分离病毒。对71名儿童的159份样本进行了研究，从81份粪便中分离出了已确定血清型的腺病毒。从35名儿童中获取了含有腺病毒颗粒的系列粪便样本，常见已识别血清型的长时间排毒现象。还观察到了不同血清型的同时感染和相继感染。36名儿童排出了使用通常用于检测腺病毒的细胞培养方法无法分离的腺病毒，其中9名儿童还排出了已确定血清型的腺病毒。在张氏结膜细胞培养中传代，可将来自14名儿童的难培养腺病毒鉴定为一种先前未识别血清型的成员。
