来自克隆cDNA的琵琶鱼和哺乳动物胰岛素mRNA核酸序列的比较。

Comparison of the nucleic acid sequence of anglerfish and mammalian insulin mRNA's from cloned cDNA's.

作者信息

Hobart P M, Shen L P, Crawford R, Pictet R L, Rutter W J

出版信息

Science. 1980 Dec 19;210(4476):1360-3. doi: 10.1126/science.7001633.

Abstract

Anglerfish (Lophius americanus) insulin complementary DNA was cloned in bacterial plasmids, and its sequence was determined. Fish insulin messenger RNA is larger (1.5 times) than the messenger RNA encoding mammalian (rat and human) insulin, in part because of a larger C peptide (an additional six amino acids or 18 nucleotides in length) but mainly because of increases in the 5' and 3' untranslated regions. Comparison of the fish, rat, and human insulin messenger RNA (from the complementary DNA) reveals that, in addition to the regions coding for the A and B peptides, sequence conservation is limited to a segment within the 5' untranslated region which may be involved in ribosomal binding, two small segments of the signal peptide, and two stretches of sequence in the 3' untranslated region.

摘要

美洲鮟鱇鱼（Lophius americanus）胰岛素互补DNA被克隆到细菌质粒中，并测定了其序列。鱼类胰岛素信使核糖核酸比编码哺乳动物（大鼠和人类）胰岛素的信使核糖核酸大（1.5倍），部分原因是C肽更大（额外六个氨基酸或18个核苷酸长度），但主要是因为5'和3'非翻译区增加。对鱼类、大鼠和人类胰岛素信使核糖核酸（来自互补DNA）的比较表明，除了编码A和B肽的区域外，序列保守性仅限于5'非翻译区内可能参与核糖体结合的一段序列、信号肽的两个小片段以及3'非翻译区的两段序列。