Bodaness R S, Zigler J S
Biochem Biophys Res Commun. 1983 Jun 15;113(2):592-7. doi: 10.1016/0006-291x(83)91767-9.
Human cataract lens crystallins are crosslinked and demonstrate a non-tryptophan blue fluorescence. We report here that exposure of lens crystallin to H2O2 within the concentration range reported for human aqueous humor, produces crosslinking of crystallin polypeptides within 10 minutes in the presence of the heme-undecapeptide from cytochrome c. Concomitantly, a blue fluorescence develops. These findings suggest the possibility that under some conditions hydrogen peroxide activation may play a role in cataractogenesis in vivo.
人类白内障晶状体中的晶状体蛋白发生交联，并呈现出非色氨酸蓝色荧光。我们在此报告，在人房水报道的浓度范围内，将晶状体蛋白暴露于过氧化氢中，在细胞色素c的血红素十一肽存在的情况下，10分钟内晶状体蛋白多肽就会发生交联。同时，会产生蓝色荧光。这些发现表明，在某些条件下，过氧化氢激活可能在体内白内障形成过程中起作用。
