Feinstein M B, Egan J J, Sha'afi R I, White J
Biochem Biophys Res Commun. 1983 Jun 15;113(2):598-604. doi: 10.1016/0006-291x(83)91768-0.
Maximal stimulation of platelets with thrombin results in a rapid increase in cytoplasmic Ca2+ (from 0.1 microM to 1-3 microM), as measured with the fluorescent intracellular Ca2+ indicator Quin-2. Prior addition of the adenylate cyclase stimulators PGD2, PGE1 or forskolin inhibited the rise in cytoplasmic Ca2+. When added after the maximal response to thrombin was attained adenylate cyclase stimulators caused a rapid fall of cytoplasmic Ca2+ back to the original "resting" level. This effect coincides with the reversal of thrombin-induced, Ca2+-dependent protein phosphorylation, and cytoskeleton assembly. It is suggested that cAMP-dependent reactions maintain low levels of cytoplasmic Ca2+ by promoting transport and/or binding of Ca2+.
用凝血酶对血小板进行最大刺激会导致细胞质Ca2+迅速增加（从0.1微摩尔升至1 - 3微摩尔），这是用荧光细胞内Ca2+指示剂喹啉-2测量得出的结果。预先添加腺苷酸环化酶刺激剂前列腺素D2、前列腺素E1或福斯高林可抑制细胞质Ca2+的升高。当在达到对凝血酶的最大反应后添加时，腺苷酸环化酶刺激剂会使细胞质Ca2+迅速降至原来的“静息”水平。这种效应与凝血酶诱导的、Ca2+依赖性蛋白磷酸化及细胞骨架组装的逆转相吻合。有人提出，cAMP依赖性反应通过促进Ca2+的转运和/或结合来维持细胞质Ca2+的低水平。
