细胞内诱导剂汞离子（Hg2+）的浓度是决定细胞中汞抗性操纵子表达的速率因素。
Intracellular inducer Hg2+ concentration is rate determining for the expression of the mercury-resistance operon in cells.
作者信息
Yu H, Chu L, Misra T K
机构信息
Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago, 60612-7344, USA.
出版信息
J Bacteriol. 1996 May;178(9):2712-4. doi: 10.1128/jb.178.9.2712-2714.1996.
Abstract
Experiments involving mercury resistance mer operon-lacZ fusions, point mutations in the mercuric ion reductase merA gene, and transcomplementation have revealed that in Hg2+-resistant cells, the inducer Hg2+ concentration is rate determining for activation of transcription. mer operon expression is activated by the presence of nanomolar concentrations of Hg2+ in liquid media only when the mercuric ion reductase function is artificially inactivated in cells, whereas cells with active mercuric ion reductase require micromolar concentrations of Hg2+ for effective induction of the operon.
摘要
涉及汞抗性mer操纵子 - lacZ融合、汞离子还原酶merA基因中的点突变以及反式互补的实验表明，在抗Hg2+的细胞中，诱导剂Hg2+浓度是转录激活的速率决定因素。仅当细胞中的汞离子还原酶功能被人为失活时，在液体培养基中纳摩尔浓度的Hg2+存在才能激活mer操纵子表达，而具有活性汞离子还原酶的细胞则需要微摩尔浓度的Hg2+才能有效诱导操纵子。
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