大肠杆菌的carB基因：一个编码氨甲酰磷酸合成酶大亚基的重复基因。
The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase.
作者信息
Nyunoya H, Lusty C J
出版信息
Proc Natl Acad Sci U S A. 1983 Aug;80(15):4629-33. doi: 10.1073/pnas.80.15.4629.
Abstract
Previous genetic and biochemical studies indicate that the carB gene of Escherichia coli codes for the large subunit of carbamoyl-phosphate synthetase (EC 6.3.5.5). We have determined the nucleotide sequence of a 4-kilobase-pair cloned fragment of E. coli DNA with genetic determinants for carB. The DNA sequence is a 3,219-nucleotide-long reading frame. The polypeptide encoded by this reading frame has been verified to be the large subunit of carbamoyl-phosphate synthetase. The gene product is similar to the large subunit in its molecular weight, amino acid composition and amino-terminal residue, and carboxyl-terminal sequence. The amino acid sequence derived from the nucleotide sequence shows a highly significant homology between the amino- and carboxyl-terminal halves of the protein. We propose that the carB gene was formed by an internal duplication of a smaller ancestral gene.
摘要
先前的遗传学和生物化学研究表明，大肠杆菌的carB基因编码氨甲酰磷酸合成酶（EC 6.3.5.5）的大亚基。我们已经确定了一段4千碱基对的大肠杆菌DNA克隆片段的核苷酸序列，该片段带有carB的遗传决定因素。该DNA序列是一个3219个核苷酸长的阅读框。由这个阅读框编码的多肽已被证实是氨甲酰磷酸合成酶的大亚基。该基因产物在分子量、氨基酸组成、氨基末端残基和羧基末端序列方面与大亚基相似。从核苷酸序列推导的氨基酸序列显示该蛋白质的氨基末端和羧基末端两半之间具有高度显著的同源性。我们提出carB基因是由一个较小的祖先基因内部重复形成的。
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The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase.大肠杆菌的carB基因：一个编码氨甲酰磷酸合成酶大亚基的重复基因。
Proc Natl Acad Sci U S A. 1983 Aug;80(15):4629-33. doi: 10.1073/pnas.80.15.4629.
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