大肠杆菌car AB基因簇中的极性模式。
Patterns of polarity in the Escherichia coli car AB gene cluster.
作者信息
Gigot D, Crabeel M, Feller A, Charlier D, Lissens W, Glansdorff N, Piérard A
出版信息
J Bacteriol. 1980 Aug;143(2):914-20. doi: 10.1128/jb.143.2.914-920.1980.
Abstract
The direction of transcription of the carAB gene cluster, which codes for Escherichia coli carbamoylphosphate synthase, was deduced from the effects of phage Mu-1 insertions in each of the two genes and from the results of ribonucleic acid-deoxyribonucleic acid hybridization experiments relating the quantity of car messenger ribonucleic acid to the location of various car mutations. The car locus appears to constitute an operon polarized from carA to carB. The levels of carA and carB products were determined in a large number of car mutants by using in vitro and in vivo complementation assays. The results obtained display strong anomalies, which are discussed in light of the conclusions described above.
摘要
编码大肠杆菌氨甲酰磷酸合成酶的carAB基因簇的转录方向，是根据噬菌体Mu-1插入这两个基因中每个基因的效应，以及通过将car信使核糖核酸的量与各种car突变的位置相关联的核糖核酸-脱氧核糖核酸杂交实验结果推导出来的。car基因座似乎构成了一个从carA到carB呈极性的操纵子。通过体外和体内互补分析，在大量car突变体中测定了carA和carB产物的水平。所获得的结果显示出强烈的异常情况，并根据上述结论进行了讨论。
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