Ropp M, Courgeon A M, Calvayrac R, Best-Belpomme M
Can J Biochem Cell Biol. 1983 Jun;61(6):456-61. doi: 10.1139/o83-061.
In vitro cultured Drosophila melanogaster cells were shown to be aerobic and several kinetic parameters of their respiration were measured. This allowed us to define experimental conditions for a transient period of anaerobiosis followed by a reexposure to normal oxygenation. This treatment, applied without any change of temperature, induced not only the heat-shock proteins, but also a new specific peptide of 27 000 daltons and a twofold increase of the maximal rate of O2 uptake. This evokes a common molecular mechanism activated either by heat or by O2, which could involve the increase of the products of oxygen reduction such as the superoxide ion.
体外培养的黑腹果蝇细胞表现出需氧特性，并对其呼吸作用的几个动力学参数进行了测量。这使我们能够确定短暂厌氧期后再暴露于正常氧合状态的实验条件。这种处理在温度无任何变化的情况下进行，不仅诱导了热休克蛋白的产生，还诱导了一种新的27000道尔顿的特异性肽的产生，并且氧气摄取的最大速率增加了两倍。这引发了一种由热或氧气激活的共同分子机制，这可能涉及氧还原产物如超氧离子的增加。
