Bhattacherjee P, Hammond B, Salmon J A, Stepney R, Eakins K E
Eur J Pharmacol. 1981 Jul 17;73(1):21-8. doi: 10.1016/0014-2999(81)90141-2.
The effects of arachidonic acid, its cyclo-oxygenase and lipoxygenase products and the synthetic chemotactic peptide, formyl-methionyl-leucyl-phenylalanine (FMLP) on leukocyte accumulation in the aqueous humour and intraocular pressure in the rabbit were studied in vivo. Substances were injected into the anterior chamber of the eyes of anaesthetised rabbits using a closed circuit perfusion system. Injection of arachidonic acid, prostaglandins E1 and E2, and the monohydroperoxy and hydroxy acids of the lipoxygenase pathway did not result in any significant accumulation of leukocytes in the anterior chamber. In contrast, FMLP and 5,12-diHETE (Leukotrine B4) resulted in significant dose dependent accumulation of leukocytes into the aqueous humour. Leukocytes appeared in the aqueous humour between 2 and 3 h after the injection of either FMLP or LTB4 and the response was maximal at 4 h. None of the lipoxygenase products tested had any effect on intraocular pressure in contrast to the profound effects observed with arachidonic acid and the E type prostaglandins. FMLP had a small but significant effect on intraocular pressure at the highest dose tested for leukocyte accumulation. These results indicate that the effects of the cyclo-oxygenase products of arachidonate metabolism are mainly vascular in the rabbit eye in contrast to the predominantly cellular effects of lipoxygenase products. Thus in the eye, the interaction of cyclo-oxygenase and lipoxygenase products of arachidonate metabolism may be important in the development of both acute and chronic ocular inflammation.
在体内研究了花生四烯酸、其环氧化酶和脂氧化酶产物以及合成趋化肽甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）对兔房水中白细胞积聚和眼压的影响。使用闭路灌注系统将物质注入麻醉兔眼的前房。注入花生四烯酸、前列腺素E1和E2以及脂氧化酶途径的单氢过氧化物和羟基酸后，前房内未出现任何明显的白细胞积聚。相反，FMLP和5,12-二氢二十碳四烯酸（白三烯B4）导致白细胞剂量依赖性地大量积聚到房水中。注入FMLP或白三烯B4后2至3小时，房水中出现白细胞，4小时时反应达到最大值。与花生四烯酸和E型前列腺素所观察到的显著影响不同，所测试的脂氧化酶产物均未对眼压产生任何影响。在测试白细胞积聚的最高剂量下，FMLP对眼压有微小但显著的影响。这些结果表明，与脂氧化酶产物主要产生细胞效应不同，花生四烯酸代谢的环氧化酶产物在兔眼中主要产生血管效应。因此，在眼睛中，花生四烯酸代谢的环氧化酶和脂氧化酶产物之间的相互作用可能在急性和慢性眼部炎症的发展中起重要作用。
