Leinert J, Simon I, Hötzel D
Int J Vitam Nutr Res. 1983;53(2):156-65.
Methods and Their Evaluation to Estimate the Vitamin B6-Status in Human Subjects. V. S-PLP-Determination: Method and Comparison of Methods. A method to determine the plasma pyridoxal 5-phosphate (PLP) basing on the enzymatic decarboxylation of 1-14C-l-tyrosine and the measurement of the radio-active 14CO2 is described. Its efficiency is discussed in comparison with other methods. High specificity, precision, accuracy, and sensitivity are demonstrated. The developed equipment allows to analyse a great number of samples and minimizes the risk of radio-active contamination. The commercial apoenzyme preparations are different in quality and require purification. Apoenzyme lots with high genuine content of PLP are useless. Storage tests at 4 degrees C, -18 degrees C, and -196 degrees C show stability of the samples for one day, 30 days, and at least 10 weeks.
评估人体维生素B6状态的方法及其评价。V. S-磷酸吡哆醛的测定：方法及方法比较。描述了一种基于1-14C-L-酪氨酸的酶促脱羧反应和放射性14CO2测量来测定血浆5-磷酸吡哆醛（PLP）的方法。与其他方法相比，讨论了其效率。证明了该方法具有高特异性、精密度、准确性和灵敏度。所开发的设备能够分析大量样本，并将放射性污染风险降至最低。市售的脱辅酶制剂质量不同，需要纯化。PLP真实含量高的脱辅酶批次并无用处。在4℃、-18℃和-196℃下的储存测试表明，样本分别可稳定保存一天（4℃）、30天（-18℃）和至少10周（-196℃）。
