Krause H M, Rothwell M R, Higgins N P
Nucleic Acids Res. 1983 Aug 25;11(16):5483-95. doi: 10.1093/nar/11.16.5483.
The early promoter of bacteriophage Mu has been identified and characterized by a nitrocellulose filter binding assay and analysis of RNA products transcribed in vitro and in vivo. A tight, heparin resistant, RNA polymerase-DNA binding site overlaps the left Mu Hind III site. In vitro [gamma 32P] ATP initiated transcription begins approximately 25 nucleotides to the right of the Hind III restriction site. Dinucleotide initiation with ApA and S1 mapping of in vitro and in vivo transcripts shows that transcription is initiated 1028 base pairs from the Mu genetic left end.
通过硝酸纤维素滤膜结合试验以及对体外和体内转录的RNA产物的分析，噬菌体Mu的早期启动子已被鉴定和表征。一个紧密的、抗肝素的RNA聚合酶-DNA结合位点与左侧Mu Hind III位点重叠。体外[γ-32P]ATP起始转录在Hind III限制位点右侧约25个核苷酸处开始。用ApA进行二核苷酸起始以及对体外和体内转录本进行S1作图表明，转录从Mu基因左端起始1028个碱基对处开始。
