van Meeteren R, Giphart-Gassler M, van de Putte P
Mol Gen Genet. 1980;179(1):185-9. doi: 10.1007/BF00268462.
Using pBR322 as a vector, three plasmids were constructed, pGP2, pGP3, and pGP7, containing respectively 5, 100, 700-950, and 1,000 base pairs derived from the immunity end of bacteriophage Mu. All three plasmids contain a functional repressor gene coding for a thermosensitive repressor. RNAs produced when the DNA of these plasmids was used as template in in vitro RNA synthesis, were analysed by hybridization to the DNA of several lambda pMu transducing phages. In spite of the differences in length of the Mu fragments all three plasmids show the same amount of Mu specific l-strand transcription. Since the repressor gene comprises at least 70% of the Mu fragments of pGP3 and pGP7, these results indicate that the repressor gene c of bacteriophage Mu is transcribed on the l-strand. Analysis of in vivo RNA from cells harboring the plasmids pGP2, pGP3, or pGP7 also indicates that the repressor gene of phage Mu is transcribed on the l-strand, as all Mu-specific RNA extracted from these cells at 28 degrees C hybridizes with the l-strand of the first 3,100 basepairs from the Mu immunity end.
以pBR322作为载体，构建了三种质粒，即pGP2、pGP3和pGP7，它们分别含有来自噬菌体Mu免疫末端的5、100、700 - 950和1000个碱基对。所有这三种质粒都含有一个编码热敏阻遏物的功能性阻遏基因。当这些质粒的DNA在体外RNA合成中用作模板时所产生的RNA，通过与几种λpMu转导噬菌体的DNA杂交进行分析。尽管Mu片段长度存在差异，但所有三种质粒都显示出相同量的Mu特异性l链转录。由于阻遏基因至少占pGP3和pGP7的Mu片段的70%，这些结果表明噬菌体Mu的阻遏基因c在l链上转录。对携带质粒pGP2、pGP3或pGP7的细胞中的体内RNA分析也表明，噬菌体Mu的阻遏基因在l链上转录，因为在28℃从这些细胞中提取的所有Mu特异性RNA都与来自Mu免疫末端的前3100个碱基对的l链杂交。
