Transcription of bacteriophage Mu. II. Transcription of the repressor gene.

Using pBR322 as a vector, three plasmids were constructed, pGP2, pGP3, and pGP7, containing respectively 5, 100, 700-950, and 1,000 base pairs derived from the immunity end of bacteriophage Mu. All three plasmids contain a functional repressor gene coding for a thermosensitive repressor. RNAs produced when the DNA of these plasmids was used as template in in vitro RNA synthesis, were analysed by hybridization to the DNA of several lambda pMu transducing phages. In spite of the differences in length of the Mu fragments all three plasmids show the same amount of Mu specific l-strand transcription. Since the repressor gene comprises at least 70% of the Mu fragments of pGP3 and pGP7, these results indicate that the repressor gene c of bacteriophage Mu is transcribed on the l-strand. Analysis of in vivo RNA from cells harboring the plasmids pGP2, pGP3, or pGP7 also indicates that the repressor gene of phage Mu is transcribed on the l-strand, as all Mu-specific RNA extracted from these cells at 28 degrees C hybridizes with the l-strand of the first 3,100 basepairs from the Mu immunity end.